Tropical Plant Research

Tropical Plant Research

An International Journal by Society for Tropical Plant Research

E-ISSN: 2349-1183 P-ISSN: 2349-9265
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2016, VOLUME 3 ISSUE 2Pages: 328-333

Modulation of cell-wall bound phenolics accumulation by shikimate pathway in yeast extract elicited fragrant roots of Hemidesmus indicus

Anish Kundu* and Adinpunya Mitra
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Abstract:
Plant cell walls contain various phenolic compounds, originated from phenylpropanoid pathway. Hemidesmus indicus is a well-known tropical Indian medicinal plant, traditionally used as herbal medicine for different ailments. Several reports have been published on its soluble phenolic constituents so far. Recently, shikimate pathway modulated biosynthesis of 2-hydroxy-4-mrthoxybenzaldehyde, a major soluble phenolic compound of H. indicus, has been demonstrated. Here we have reported three major cell-wall bound phenolic compounds (4-hydroxybenzoic acid, 4-coumaric acid and ferulic acid) in the fragrant Hemidesmus indicus root. We described their accumulation pattern in quantitative manner by high performance liquid chromatographic analysis before and after elicitation with yeast extract and correlation of their accumulation with shikimate pathway by inhibition with glyphosate treatment.
Chromatographic analysis: A, RP-HPLC chromatograms of authentic standards of different phenolic compounds. Peak identity: 1. 4-Hydroxybenzoic acid, 2.vanillic acid, 3.vanillin, 4. 4-hydroxybenzaldehyde, 5.4-coumaric acid, 6. trans-ferulic acid; B, RP-HPLC chromatograms of cell-wall bound fractions. Peak identity: 1. 4-hydroxybenzoic acid, 2. 4- coumaric acid, 3.ferulic acid. UV spectra are shown in inset. The unidentified peak is denoted by ‘*’.

Fig.: Chromatographic analysis: A, RP-HPLC chromatograms of authentic standards of different phenolic compounds. Peak identity: 1. 4-Hydroxybenzoic acid, 2.vanillic acid, 3.vanillin, 4. 4-hydroxybenzaldehyde, 5.4-coumaric acid, 6. trans-ferulic acid; B, RP-HPLC chromatograms of cell-wall bound fractions. Peak identity: 1. 4-hydroxybenzoic acid, 2. 4- coumaric acid, 3.ferulic acid. UV spectra are shown in inset. The unidentified peak is denoted by ‘*’.


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