Evaluation of in-vitro cytotoxic and thrombolytic activity of methanolic extract of Xanthium indicum L.
Muhammad Sazzad Hossain, Md. Hossan Sakib*, Mohammad Shahin Alam, Ahsan Ullah, Sadequr Rahman and Limon Kanti Shill
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Abstract: The present study was designed to investigate the cytotoxic and thrombolytic activity of methanolic extract of fruits of Xanthium indicum. Methanolic extract of Xanthium indicum, was used to evaluate its cytotoxicity in Brine shrimp lethality bioassay where vincristine sulphate was used as standard drug. Thrombolytic effect of the fraction was investigated in clot lysis experiment. In Brine shrimp lethality bioassay, LC50 value of the extract was 30.90 μg.ml-1 and vincristine sulphate served as the positive control showed LC50 value 10.51 μg.ml-1. The extract exerted 48.47% lysis of the blood clot in thrombolytic activity test while 48.83% and 13.82% lysis were obtained for positive control (streptokinase) and negative control respectively. Compared to vincristine sulphate. It is evident that the methanolic extract of fruits of Xanthium indicum was cytotoxic. So, the extract possessed considerable thrombolytic activity. Which compounds is responsible for the present pharmacological actions and to know their mechanism of action, extensive pharmacological and phytochemical experiments are essential.
Fig.: Determination of LC50 value for fraction of Xanthium indicum leaves from linear correlation between log concentrations versus percentage of mortality.