Tropical Plant Research
An International Journal by Society for Tropical Plant Research
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2016, VOLUME 3 ISSUE 3Pages: 634-641
Isolation and characterization of lectin from the leaves of Euphorbia tithymaloides (L.)
Aruna A. Jawade, Shubhangi K. Pingle*, Rajani G. Tumane, Anvita S. Sharma, Archana S. Ramteke and Ruchika K. Jain
Abstract:
Lectins or glycoproteins from the leaves of Euphorbia tithymalo were isolated after screening of different flora from Central India. The crude extract of leaves was dialyzed andammonium sulfate precipitation done and followed by dialysis for the purification of lectins. Protein concentration in the purified extract was 10 mg.ml-1 measured by Biuret Method. The purified lectins were able to agglutinate human erythrocytes of ABO blood group system. Agglutination was also visible with animal erythrocytes. Lectin of ET was Galactose/Lactose specific and shows maximum activity at pH-7 and temperature between 40–60º C. Molecular weight of purified extract of ET was determined by 1D- SDS Polyacrylamide gel electrophoresis which was found to be 70.24, 28.53 and 14.68 kDa.
Lectins or glycoproteins from the leaves of Euphorbia tithymalo were isolated after screening of different flora from Central India. The crude extract of leaves was dialyzed andammonium sulfate precipitation done and followed by dialysis for the purification of lectins. Protein concentration in the purified extract was 10 mg.ml-1 measured by Biuret Method. The purified lectins were able to agglutinate human erythrocytes of ABO blood group system. Agglutination was also visible with animal erythrocytes. Lectin of ET was Galactose/Lactose specific and shows maximum activity at pH-7 and temperature between 40–60º C. Molecular weight of purified extract of ET was determined by 1D- SDS Polyacrylamide gel electrophoresis which was found to be 70.24, 28.53 and 14.68 kDa.
Fig.: Isoelectric focusing of lectins of ET on 2-D PAGE.
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