2017, VOLUME 4 ISSUE 1Pages: 13-19
In Vitro antioxidant, antibacterial and phytochemical screening of Cochlospermum religiosum (L.) Alston - A potent medicinal plant
Pooja Ponnamma, G. Manasa, M. S. Sudarshana, M. Murali and C. Mahendra*
*University of Mysore, Department of Studies in Botany, Manasagangotri, Mysore-570006, Karnataka, India
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The work is undertaken to evaluate the preliminary phytochemicals, antibacterial and antioxidants activity of Cochlospermum religiosum leaf extracts with three solvents via chloroform, ethyl acetate and methanol based on polarity index. The antibacterial activity was assessed against five bacterial pathogens like Escherichia coli, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus and Pseudomonas aeruginosa by well diffusion assay. Among the tested pathogens, the maximum zone of inhibition was observed against E. coli (26 mm) followed by P. aeruginosa (23 mm) in ethyl acetate extracts compare to other solvent extracts. Phytochemical analysis also revealed the presence of various pharmaceutically active secondary metabolites like alkaloids, phenolic, flavonoids, saponins, carbohydrates, proteins, glycosides, sterols, etc. Antioxidant activity was determined by DPPH scavenging, total phenolic and phospho-molybdenum method. In DPPH assay, ethyl acetate extract was found to be the most effective. Similarly, total phenols and phospho-molybdenum assay the methanol extracts was found to contained good sources of antioxidants. The outcomes of the present study specified the plant possess various potentially active secondary metabolites which help for the developing pharmaceuticals, especially antioxidant and antimicrobial drugs.