Tropical Plant Research
An International Journal by Society for Tropical Plant Research
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2017, VOLUME 4 ISSUE 3Pages: 433-440
Isolation and characterization of hemagglutinins from Clerodendrum phlomidis L.f.
Aaditi S. Khagar, Prerna S. Khagar, Shubhangi K. Pingle*, Rajani G. Tumane, Aruna A. Jawade and Shraddha Jaiswal
*National Institute of Miners’ Health, JNARDDC Campus, Wadi Nagpur-440023, Maharashtra, India
Abstract:
Lectins from the leaves, fruit, calyx and stem of Clerodendrum phlomidis plant were isolated after screening of total 54 plants from central India. The crude extract of leaves, fruit, calyx and stem of C. phlomidis plant was purified by ammonium sulfate precipitation, followed by dialysis. The isolated lectins of C. phlomidis plant were able to agglutinate human as well as dog, hen, goat mouse, mice and fish erythrocytes. The isolated lectins were found to be lactose specific. The pH stability of C. phlomidis leaves lectins was between 4–10 pH, for fruit lectins it was 5–7 pH, and for calyx and stem lectins it was found stable in between 4 and 8 pH. The calyx and stem lectins were active until 60ºC, fruit has shown activity upto 50ºC and in case of leaves, it was found maximum 40ºC. Beyond their respective optimum temperature and pH range, all the lectins were unable to agglutinate erythrocytes. Hemagglutination activity of C. phlomidis plant lectins was also checked in the presence of different metal ions and it was found that it was partially inhibited by aluminium metal ion.
Lectins from the leaves, fruit, calyx and stem of Clerodendrum phlomidis plant were isolated after screening of total 54 plants from central India. The crude extract of leaves, fruit, calyx and stem of C. phlomidis plant was purified by ammonium sulfate precipitation, followed by dialysis. The isolated lectins of C. phlomidis plant were able to agglutinate human as well as dog, hen, goat mouse, mice and fish erythrocytes. The isolated lectins were found to be lactose specific. The pH stability of C. phlomidis leaves lectins was between 4–10 pH, for fruit lectins it was 5–7 pH, and for calyx and stem lectins it was found stable in between 4 and 8 pH. The calyx and stem lectins were active until 60ºC, fruit has shown activity upto 50ºC and in case of leaves, it was found maximum 40ºC. Beyond their respective optimum temperature and pH range, all the lectins were unable to agglutinate erythrocytes. Hemagglutination activity of C. phlomidis plant lectins was also checked in the presence of different metal ions and it was found that it was partially inhibited by aluminium metal ion.
Fig.: Hemagglutinationtiter of partially purified extract of Clerodendrum phlomidis L.f.:A, Leaves; B, Calyx; C, Fruits; D, Stem.
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