Tropical Plant Research
An International Journal by Society for Tropical Plant Research
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2019, VOLUME 6 ISSUE 1Pages: 18-23
Enhanced rhizome induction and fast regeneration protocol in liquid culture of Dendrocalamus longispathus Kurz: A single step culture
Papori Phukan Borpuzari* and Narendra Singh Bisht
*Rain Forest Research Institute, Jorhat-785001, Assam, India
Abstract:
The present experimental study was aimed for in-vitro regeneration through nodal culture of Dendrocalamus longispathus an important bamboo species of north-eastern region. Single auxillary buds were cultured in different concentration of BAP and Kn incorporated media for bud breaking and shoot regeneration. Effect of collection period and type of explants is a major impact on bud breaking. Single step plantlet regeneration has been achieved in the liquid basal medium Murashigs and Skoogs (MS) with BAP 1.0 + Kn 1.0 shows best regeneration of 6 to 8 numbers of shoots within 3 weeks of culture. Both inoculated intact node and cut node cultures produced shoots and rhizomes during subcultures. Increased incubation period up to 11 weeks with serial sub culture produced simultaneous roots and rhizomes in the cultured media containing BAP 1.0 + Kn 1.0. Culture response of 90% healthy rooted plantlets has been established outside the lab condition. The whole experiment completed within 12 weeks of culture incubation. Good growth of established in-vitro plantlets in field of FRCBR, Aizwal is observed after one year.
The present experimental study was aimed for in-vitro regeneration through nodal culture of Dendrocalamus longispathus an important bamboo species of north-eastern region. Single auxillary buds were cultured in different concentration of BAP and Kn incorporated media for bud breaking and shoot regeneration. Effect of collection period and type of explants is a major impact on bud breaking. Single step plantlet regeneration has been achieved in the liquid basal medium Murashigs and Skoogs (MS) with BAP 1.0 + Kn 1.0 shows best regeneration of 6 to 8 numbers of shoots within 3 weeks of culture. Both inoculated intact node and cut node cultures produced shoots and rhizomes during subcultures. Increased incubation period up to 11 weeks with serial sub culture produced simultaneous roots and rhizomes in the cultured media containing BAP 1.0 + Kn 1.0. Culture response of 90% healthy rooted plantlets has been established outside the lab condition. The whole experiment completed within 12 weeks of culture incubation. Good growth of established in-vitro plantlets in field of FRCBR, Aizwal is observed after one year.
Fig.: Rhizome induction and plant regeneration of Dendrocalamus longispathus (Kurz): A, Shoot multiplication; B, Rooting of regenerated shoots; C, Number rhizome buds and rooting; D–G, Rooted rhizome of intact node explants; H–I, Rooted rhizomes of cut node explants; J, Deflasked plantlets after 11weeks of culture in the shoot regeneration media; K, Hardening under shaded area; L, Plants after field establishment.
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