Tropical Plant Research
An International Journal by Society for Tropical Plant Research
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2017, VOLUME 4 ISSUE 1Pages: 71-76
Callus induction and organogenesis from Tinospora formanii Udayan and Pradeep: A rare endemic plant
Sheema Dharmapal P.*, M. Najla, E. S. Swetha, P. S. Udayan and K. K. Elyas
*Department of Botany, Sree Krishna College, Guruvayur, Ariyannur P.O., Thrissur-680102, Kerala, India
Abstract:
Tinospora is a genus within the family Menispermaceae renowned for its medicinal properties. The present study was undertaken to develop an effective protocol for optimum callus induction and organogenesis in Tinospora formanii, a rare endemic plant reported from Western Ghats of Kerala. Data of number of days required for callus induction, callus induction percentage, callus morphology, callus fresh weight and number of days required for shoot regeneration were recorded. Nodal segments from one year old plants were used as explants for callus induction and shoot regeneration. After proper treatment of explants with surface sterilants, 70% (v/v) ethyl alcohol and 0.1% (w/v) mercuric chloride (HgCl2), it was transferred to Murashige and Skoog’s (MS) medium supplemented with different concentration of 6-Benzylaminopurine (0.5, 1.0, 2.0, 3.0 and 5.0 and 8.0 mg.L-1) with 1-Naphthaleneacetic acid (0.2 mg.L-1). All these hormonal combinations gave callus. Maximum callus induction (96%) was observed on medium containing 1.0 mg.L-1 6- Benzylaminopurine (BAP) with 1-Naphthaleneacetic acid (NAA) 0.2 mg.L-1. Number of days taken for callus induction in this medium was 5±0.28. Organogenic creamy brown friable callus developed. The fresh weight of the callus was 4.75±0.08 gm after 60 days of culture. Small green shoot buds developed from this callus after 65 days of culture which developed into shoots of about 2.5–3.0 cm length.
Tinospora is a genus within the family Menispermaceae renowned for its medicinal properties. The present study was undertaken to develop an effective protocol for optimum callus induction and organogenesis in Tinospora formanii, a rare endemic plant reported from Western Ghats of Kerala. Data of number of days required for callus induction, callus induction percentage, callus morphology, callus fresh weight and number of days required for shoot regeneration were recorded. Nodal segments from one year old plants were used as explants for callus induction and shoot regeneration. After proper treatment of explants with surface sterilants, 70% (v/v) ethyl alcohol and 0.1% (w/v) mercuric chloride (HgCl2), it was transferred to Murashige and Skoog’s (MS) medium supplemented with different concentration of 6-Benzylaminopurine (0.5, 1.0, 2.0, 3.0 and 5.0 and 8.0 mg.L-1) with 1-Naphthaleneacetic acid (0.2 mg.L-1). All these hormonal combinations gave callus. Maximum callus induction (96%) was observed on medium containing 1.0 mg.L-1 6- Benzylaminopurine (BAP) with 1-Naphthaleneacetic acid (NAA) 0.2 mg.L-1. Number of days taken for callus induction in this medium was 5±0.28. Organogenic creamy brown friable callus developed. The fresh weight of the callus was 4.75±0.08 gm after 60 days of culture. Small green shoot buds developed from this callus after 65 days of culture which developed into shoots of about 2.5–3.0 cm length.
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