2021, VOLUME 8 ISSUE 1Pages: 81-94
Genetic diversity of Ocimum species (Scent leaf) landraces from South Nigeria using inter-simple sequence repeat (ISSR) markers
Kafilat Abiodun Odesola, Edidiong Nkiruka Essien, Kayode Ezekiel Ogunsola, David Okeh Igwe and Omena Bernard Ojuederie*
*Department of Biological Sciences, Biotechnology Unit, Faculty of Science, Kings University, PMB 555, Odeomu, Osun State, Nigeria
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Ocimum species, commonly known as scent leaf or basil are aromatic or scented shrubs that belong to the Lamiaceae family. This study investigated the genetic diversity of twenty-five scent leaf genotypes using ten Inter-Simple Sequence Repeat (ISSR) markers. Seeds of 25 Ocimum genotypes were obtained from five different Local Government Areas in six southern states of Nigeria. The ten ISSR primers generated a total of 679 bands with 629 bands been polymorphic (92.64%). Primer UBC813 gave the highest number of polymorphic bands (143). The unweighted pair-group method with arithmetic average cluster analysis grouped the 25 genotypes of the scent leaves into six major clusters with a dissimilarity distance from 0.015 to 0.288. Genotypes Udu-DT and Ikp-EDO were the most closely related at a dissimilarity coefficient of 0.015. The polymorphic information content values ranged from 0.381 (UBC834) to 0.913 (UBC813) with a mean of 0.677. UBC813 was the most polymorphic and informative ISSR marker for diversity studies on scent leaves. An extremely high genetic diversity was observed across the genomic loci of the genotypes which showed the effectiveness of ISSR markers in determining the extent of genetic variability in Ocimum species. Among the genotypes, Uyo-AKS had the highest values of genetic parameters including the effective number of alleles (1.4511), Nei’s gene diversity (0.3108) and Shannon’s information index (0.4898). The findings of this study gave valid procedures for the management of Ocimum genetic resources which will enhance the utilization, genetic conservation, and improvement of the species in Nigeria.