2017, VOLUME 4 ISSUE 2Pages: 351-357
Evaluation of antimicrobial activity of Phellinus linteus (Berk. & M.A Curtis.) with their wild collections from Western Ghats of India
Jayashree K. Kodiyalmath* and Krishnappa M.
*Department of P.G. Studies and research in Applied Botany, Kuvempu University, Shankaraghatta-577451, Shimoga, Karnataka, India
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A survey was conducted in Sagar taluk Shivamogaa district, in the month of June to August 2015 to collect the Phellinus linteus from the wood logs of Jack fruit. The samples were dried and powdered, subjected to extraction using petroleum ether, chloroform and methanol successively by soxhlet method. Extracts were tested for secondary metabolites and showed the presence of alkaloids, phenols, sterols and flavonoids. The sporocarp extract was screened for their antimicrobial activity against different human pathogens like Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, Candida albicans, Chrysosporium keratinophilum, Pencillium chrysogenum, Aspergillus niger and plant pathogens like Xanthomonas campestris and Agrobacterium tumefaciens using agar well diffusion method. The methanol extracts showed maximum activity followed by chloroform, petroleum ether. The inhibition effects on fungi were low compared to bacteria. GCMS analysis showed the presence of ergosterol. The study reveals that Phellinus linteus showed good antimicrobial activity against Candida albicans and Staphylococcus aureus.
Fig.: Antifungal activity of Phellinus linteus (Berk.
& M.A Curtis.) at 100% concentration of different solvent extract. [Ca= Candida albicans, Cm= Chrysosporium
merdarium, Tr= Trichophyton rubrum, Ck= Chrysosporium
keratinophilum, Fs= Fusarium solani, Pc= Penicillium
chrysogenum, Af= Aspergillus flavus, An=Aspergillus niger]