Tropical Plant Research

Tropical Plant Research

An International Journal by Society for Tropical Plant Research

ISSN (E): 2349-1183 ISSN (P): 2349-9265
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2017, VOLUME 4 ISSUE 3Pages: 480-485

In vitro propagation of Pueraria tuberosa (Roxb. ex Willd.) DC.

Bindu T. K. *, Sheema Dharmapal P., P. S. Udayan, A. V. Raghu and Rahul R. Nair
*Post Graduate Department of Botany & Research Centre, Sree Krishna College, Guruvayur, Ariyannur P. O., Thrissur-680102, Kerala, India
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Abstract:
Pueraria tuberosa, commonly known as Indian kudzu or 'Vidari' is an important medicinal plant belonging to the family Fabaceae. The tubers of this plant are an important constituent in many Ayurvedic formulations as a restorative tonic, immune booster and antiageing. Annual demand of Vidari by the Ayurvedic industry is 135 tonnes and industry is facing a severe scarcity of this raw material. Micropropagation technology offers large-scale production of disease-free, quality planting materials for pharmaceutical industries. A successful protocol for the in vitro propagation of P. tuberosa has been achieved by using nodal segments as explants. Multiple shoot induction and proliferation was obtained in Murashige and Skoog medium supplemented with 0.5 mg.L-1 BAP, 0.5 mg.L-1 KN and 2% glucose. MS medium with 1.5 mg.L-1 KNfavoured maximum shoot elongation. A maximum number of roots with the highest percentage of rooting was observed on half strength MS media supplemented with 0.5 mg.L-1 IBA. Elongated shoots in half strength basal MS medium induced roots in 14 days of culture. Regenerated shoots with well-developed roots were successfully hardened with 80% survival.
<strong>A,</strong> Shoot bud initiation of Pueraria tuberosa from nodal explants on MS medium supplemented with 0.5 mg.L<sup>-1 </sup>BAP and 0.5 mg.L<sup>-1 </sup>KN; <strong>B–C,</strong> Multiple shoot induction after 40 days of culture on 0.5 mg.L<sup>-1 </sup>BAP and 0.5 mg.L<sup>-1 </sup>KN; <strong>D,</strong> Shoot elongation on MS medium with 1.5 mg.L<sup>-1 </sup>KN; <strong>E,</strong> In vitro rooting on half srength MS with 0.5 mg.L<sup>-1 </sup>IBA; <strong>F,</strong> Acclimatization.

Fig.: A, Shoot bud initiation of Pueraria tuberosa from nodal explants on MS medium supplemented with 0.5 mg.L-1 BAP and 0.5 mg.L-1 KN; B–C, Multiple shoot induction after 40 days of culture on 0.5 mg.L-1 BAP and 0.5 mg.L-1 KN; D, Shoot elongation on MS medium with 1.5 mg.L-1 KN; E, In vitro rooting on half srength MS with 0.5 mg.L-1 IBA; F, Acclimatization.


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