2017, VOLUME 4 ISSUE 2Pages: 210-224
Nitrogen level affects growth and reactive oxygen scavenging of fenugreek irrigated with wastewater
Saima Kausar*, Shahla Faizan and Irfana Haneef
*Department of Botany, Jhunjhunwala Degree College, Faizabad, Uttar Pradesh, India
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Today, due to the constraint in availability of the freshwater for irrigation, wastewater is being used for irrigation of agriculture fields. Wastewater contain plant nutrients that favour crop growth but leave a burden of heavy metals which can enter the food chain and is a cause of great concern. This research work aimed at evaluating the potential utilization of wastewater as an alternative source of water and nitrogen (N) for fenugreek. For this purpose, a pot experiment was conducted with wastewater and four doses of nitrogen (0, 20, 40 and 60 kg.ha-1) to observe a comparative effect on growth and biochemical characteristics using fenugreek as the test crop. Wastewater and soil used in the experiment were analyzed for their physicochemical properties. Results indicate that wastewater was richer in essential plant nutrients but contained some heavy metals in amounts well below the permissible limits for its use as irrigation water. Use of wastewater with 20 kg.N.ha-1 improved growth, proline and antioxidant enzymes of the plant as compared to application of groundwater with no fertilizer. Lipid peroxidation increased with wastewater but decreased with the increasing nitrogen doses, so the best combination (WW×N20) has negative impact on this parameter. These results concluded that wastewater can efficiently substitute groundwater and also works as a nutrient source whereas application of nitrogen could provide protection against the oxidative stress by increasing the antioxidant protective system.
Fig.: Linear regression curve of fenugreek showing correlation of proline with catalase, peroxidase and superoxide dismutase at 60 DAS.