Tropical Plant Research
An International Journal by Society for Tropical Plant Research
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2021, VOLUME 8 ISSUE 3Pages: 210-216
In vitro cytological studies of Passiflora vitifolia Kunth an important medicinal and ornamental plant
K. P. Chandana, N. K. Hemanth Kumar*, H. S. Prithviraj and Shobha Jagannath
*Plant Tissue Culture Laboratory, Department of Studies in Botany, University of Mysore, Manasagangothri, Mysore, Karnataka, India
Abstract:
The study was carried out to determine the in vitro cytological behaviour of callus cultures of Passiflora vitifolia member of Passifloraceae. The callus cultures were raised using stem and leaf explants on MS medium supplemented with different growth regulators of axuin (2,4-D, IAA and NAA) and cytokinin (BAP and Kn.) and the combination of auxins with cytokinin (NAA + BAP) and (NAA + Kn.). Best callusing was achieved in the leaf and stem explants in NAA and 2-4,D at 1.5 mg l-1. Cytogenetic analysis of the callus was carried out for primary callus culture as well as 4-month-old callus culture. The chromosome number showed wide range of variation, however, the majority of the cells were observed to be diploid (2n = 18) in nature. The most observed cell and chromosomal irregularities were polyploid cells, enucleated cell, binucleatedcell, chromosomal bridges were found to be more common. The composition and concentrations of media were found to effect on chromosomal instability and in turn, these observations showed that genetic variations may arise during callogenesis.
The study was carried out to determine the in vitro cytological behaviour of callus cultures of Passiflora vitifolia member of Passifloraceae. The callus cultures were raised using stem and leaf explants on MS medium supplemented with different growth regulators of axuin (2,4-D, IAA and NAA) and cytokinin (BAP and Kn.) and the combination of auxins with cytokinin (NAA + BAP) and (NAA + Kn.). Best callusing was achieved in the leaf and stem explants in NAA and 2-4,D at 1.5 mg l-1. Cytogenetic analysis of the callus was carried out for primary callus culture as well as 4-month-old callus culture. The chromosome number showed wide range of variation, however, the majority of the cells were observed to be diploid (2n = 18) in nature. The most observed cell and chromosomal irregularities were polyploid cells, enucleated cell, binucleatedcell, chromosomal bridges were found to be more common. The composition and concentrations of media were found to effect on chromosomal instability and in turn, these observations showed that genetic variations may arise during callogenesis.
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