Tropical Plant Research

Tropical Plant Research

An International Journal by Society for Tropical Plant Research

ISSN (E): 2349-1183 ISSN (P): 2349-9265
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2023, VOLUME 10 ISSUE 1Pages: 28-45

Phytochemical study and anti-sickling, anti-inflammatory, antioxidant and cytotoxic activities of Uvariodendron molundense (Annonaceae) bark

Ruphin Djolu Djoza, Nathan Bulaba Majambu, Mardoché Monga Semine, Colette Masengo Ashande, Monizi Mawunu, Emmanuel Kitete Mulongo, Pius Mpiana Tshimankinda and Koto-Te-Nyiwa Ngbolua*
*Department of Biology, Faculty of Science and Technology, University of Kinshasa, Kinshasa, Democratic Republic of Congo
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Abstract:
The present study showed that the bark of Uvariodendron molundense contains characteristic histological elements such as fibre fragments, epidermal cell fragments, hexagonal isodiametric epidermal cells, hexagonal epidermal cells, polyhedral cells and crystalline fibre fragments. It also contains tannins, saponosides, flavonoids, phenolic acids, iridoids, anthocyanins, anthrones, anthraquinones and terpenes. The total polyphenol content was 419.514±1.666 mg EAG g-1 extract and the total flavonoid content was 2.510±0.348 mg EQ g-1 extract. The results also shown that the anti-free radical activity of the extract derived from percolation is greater than that of the decoctation extract and that U. molundense bark are not cytotoxic (%Hemolysis <50 at 1000 µg ml-1). These leaves have anti-inflammatory properties. However, this activity is low for the percolation-derived extract (%I = 49.287±7.180%) and average for the decoction derived extract (%I = 51.928±5.882%). Both decoction and percolation-derived extracts of Uvariodendron molundense species displayed good antisickling properties in vitro. The results of molecular docking indicate that Eugenol (which is the major component of essential oil of Uvariodendron genus) forms two hydrogen bonds with haemoglobin S [F103(F)O; G107(F)CA], three hydrogen bonds with nuclear factor NF-kB [E346(F)0E2; Y379(F)O; Y379(F)N] and a single hydrogen bond with myeloperoxidase [M(A)O]. Based on molecular modelling, we can therefore hypothesise that this medicinal plant acts by inhibiting haemoglobin S, nuclear factor NF-kB and myeloperoxidase respectively. Study of the interaction of this compound/ligand (Eugenol) with three receptors: haemoglobin S (ΔG = -5.91±0.21 kcal), NF-kB (ΔG = -4.98±0.22 kcal) and Myeloperoxidase (ΔG = -5.85±0.29 kcal) indicates the formation of a thermodynamically stable complex (ΔG <0). A more in-depth phytochemical study of U. molundense bark is therefore desirable in order to identify the biologically active compounds.
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