Tropical Plant Research

Tropical Plant Research

An International Journal by Society for Tropical Plant Research

ISSN (E): 2349-1183 ISSN (P): 2349-9265
Journal is inviting manuscripts for its coming issue. Contact us for more details.
2023, VOLUME 10 ISSUE 1Pages: 46-52

High-Performance Liquid Chromatography: Validation of bioactive compounds present in Ashwagandha extract

Neha Shukla, Krupali Goswami, Sanjeev Bhasin, Pawan Pal and Prashant Wagh*
Innovation and Knowledge Center, Ashapura Minechem Limited, Bhuj, Gujarat, India
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Abstract:
The phytochemical constituents of the Ashwagandha include 40 types of Withanolides, and 12 types of alkaloids. Glycowithanolides and Withaferin are the compounds have a wide range of medicinal properties such as anticancer, anti-inflammatory, antiviral and angiogenic effects. In this present investigation, suitable extraction method of Withanolides was seized and In-House Performance Liquid Chromatography (RP-HPLC) analysis method for targeted eight Withanolides of Withania somnifera was developed. The mobile phase consisted of 0.1% ortho-phosphoric acid in water and acetonitrile with gradient elution. Photodiode array detection was used to profile the composition and quantification of Withanolides. The method described good resolution and easily applied to the separation and identification of Withanolides.
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